Microscopy
Microscopy Resource Rooms 2215 – 2219:
The CORE provides training and access to advanced light microscopes customized for tissue and live cell experiments. Members of the CORE also provide technical support during business hours (8:30 am – 5:30 pm).
Microscopes:
Zeiss Axio Observer with Apotome with 5x, 10x, 20x, 40x, and 63x oil objectives for wide-field fluorescence and brightfield imaging with Z-stack option
Leica DMIRB Microscope for fluorescence, phase, and brightfield imaging with automated soft agar colony program
Nikon TE 2000-E Confocal Microscope with 4x, 10x, 20x, 40x oil, 60x water, and 100x oil objectives for DAPI, FITC, GFP, Texas Red, Cy2, Cy3, and Cy5 imaging
Zeiss Discovery V20 Microscope for manual dissection with 0.5x, 1.0x, and 3.5 objections with a zoom feature for higher magnification.
- Large object field allows entire slide mount or organism to be viewed
Zeiss Palm Microbeam for laser microdissection to isolate homogenous, morphologically-identified or fluorescently-labelled samples for DNA, RNA, or protein analysis
Tips for Quality Images:
https://jcs.biologists.org/content/122/6/753
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805368/
https://sciencellonline.com/blog/how-to-take-quality-microscopy-pictures/
http://www.enzolifesciences.com/science-center/technotes/2015/june/10-tips-for-high-quality-images-by-cellular-microscopy/